Plasmid

Part:BBa_K4077003:Design

Designed by: Himanshu Ranjan, Kavya Prince   Group: iGEM21_NIT_Warangal   (2021-10-17)


Modified Pichia Pastoris Plasmid (pPIC9k)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1222
    Illegal XbaI site found at 3703
    Illegal PstI site found at 971
    Illegal PstI site found at 6295
    Illegal PstI site found at 7536
    Illegal PstI site found at 10091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1222
    Illegal NheI site found at 3464
    Illegal NheI site found at 4411
    Illegal PstI site found at 971
    Illegal PstI site found at 6295
    Illegal PstI site found at 7536
    Illegal PstI site found at 10091
    Illegal NotI site found at 2905
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1222
    Illegal BglII site found at 1
    Illegal BglII site found at 2257
    Illegal BglII site found at 2560
    Illegal BglII site found at 8545
    Illegal BamHI site found at 938
    Illegal XhoI site found at 1192
    Illegal XhoI site found at 7380
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1222
    Illegal XbaI site found at 3703
    Illegal PstI site found at 971
    Illegal PstI site found at 6295
    Illegal PstI site found at 7536
    Illegal PstI site found at 10091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1222
    Illegal XbaI site found at 3703
    Illegal PstI site found at 971
    Illegal PstI site found at 6295
    Illegal PstI site found at 7536
    Illegal PstI site found at 10091
    Illegal AgeI site found at 2971
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5751
    Illegal BsaI site found at 7852
    Illegal BsaI.rc site found at 2286
    Illegal BsaI.rc site found at 9917
    Illegal SapI site found at 8834


Design Notes

No such special design consideration.


Source

Snapgene

References